In vitro inhibition of lens epithelial cell growth by continuous wave Nd:YAG laser.
نویسندگان
چکیده
Bovine lens epithelial cells were suspended in MEM medium and subjected to continuous wave, low power, pulsed neodymium:yttrium-aluminum-garnet (Nd:YAG) laser irradiation. The temperature of each suspension was maintained at 36 degrees C. Laser applications ranged from 1 to 10 watts and from 100 to 2000 seconds, but the total dose to each of the epithelial cell suspension was 2000 J. Six to thirty-nine percent of the cells were dead immediately after irradiation. Surviving cells, cultured for 15 days, showed decreased attachment and failed to grow. These preliminary results suggest that the Nd:YAG laser may be used during cataract surgery to prevent subsequent lens epithelial cell proliferation and the resulting vision reduction and glare.
منابع مشابه
Five-year incidence of Nd:YAG laser capsulotomy and association with in vitro proliferation of lens epithelial cells from individual specimens: a case control study
BACKGROUND The aims of this study were to determine the 5-year incidence of posterior capsule opacification (PCO) requiring Nd:YAG laser capsulotomy in a representative mixed cohort of cataract patients, to determine risk factors for PCO and to investigate possible association with growth of human lens epithelial cells (HLEC) in vitro. METHODS Pieces of the anterior lens capsule and adhering ...
متن کاملA Comparison of Six Ultrasound Stimulation Types on Pseudomonas Aeruginosa Growth in Vitro
Background: This work evaluated the efficiency of common ultrasound stimulation (U.S.S) types on bacterial growth in vitro using clinically relevant conditions.Objective: To estimate different frequencies ultrasound bactericidal ability on bacteria in bacteria of Pseudomonas Aeruginosa. Methods: Six types of U.S.S (continuous wave, 7w/cm2, 20 KHz; continuous wave, 35w/0.8L, 40 KHz; continuous ...
متن کاملComparison between Peeling with Focal Trichloroacetic Acid and Quasi-Continuous Frequency-Doubled Nd:YAG (532 nm) Laser in the Treatment of Freckles
Background: Benign pigmented lesions, including freckles, are common and various treatment modalities including lasers and peeling have been evaluated for their effectiveness in their treatment.Objective: To compare the clinical effects of focal peeling with Trichloroacetic Acid (TCA) and Nd:YAG (532 nm) laser on freckles.Methods: Twenty patients with freckles were treated with foca...
متن کاملPosterior capsular opacification: a problem reduced but not yet eradicated.
Posterior capsular opacification (PCO) is the most frequent complication of cataract surgery. Advances in surgical techniques, intraocular lens materials, and designs have reduced the PCO rate, but it is still a significant problem. The only effective treatment for PCO, Nd:YAG laser capsulotomy carries vision-related complications and risks and puts a significant financial burden on the health ...
متن کاملOptical Limiting Properties of Colloids Enhanced by Gold Nanoparticles Based on Nonlinear Refraction for Cw Laser Illumination
In this work, thermo-optical properties of gold nanoparticle colloids are studied using continuous wave (CW) laser irradiation at 532 nm. The nanoparticle colloids are fabricated by 18 ns pulsed laser ablation of pure gold plate in the distilled water. The formation of the nanoparticles has been evidenced by optical absorption spectra and transmission electron microscopy. The nonlinear optical ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Investigative ophthalmology & visual science
دوره 30 4 شماره
صفحات -
تاریخ انتشار 1989